二代测序无提取FFPE直接建库试剂盒操作流程
产品信息
1. TargetPlex NGS DNA Library Kit for Illumina
2. TargetPlex NGS DNA Library Kit for Ion Torrent
3. FFPE-Direct DNA Library Prep Kit for Illumina NGS
4. FFPE-Direct DNA Library Prep Kit for Ion Torrent NGS
5. HiFi PCR Master Mix For DNA library Amplification
6. Ultra HiFi PCR Master Mix for FFPE Direct Amplification
7. Illumina Library Amplification Primer Mix
8. 16S Metagenomics NGS Primer Pools
9. 16S Metagenomics NGS Primer Pools
10. 16S Metagenomics Library Kit
11. TargetPlex NGS NSCLC DNA Library Kit for Illumina
12. TargetPlex NSCLC NGS DNA Library Kit for Ion Torrent
13. TargetPlex NSCLC Primer Panel
14. 5X TargetPlex CHS Primer Pool
15. Illumina 16S NGS Library Kit (Two-Step PCR Method)
16. cfDNA NGS Library Kit for Illumina
17. cfDNA NGS Library Kit for Ion Torrent
18. CD Index Adaptors for Illumina NGS 1-8 Kit
19. CD Index Adaptors for Illumina NGS 1-16 Kit
20. CD Index Adaptors for Illumina NGS 1-96 Kit
21. Barcode Adaptors for Ion Torrent NGS 1-8 Kit
22. Barcode Adaptors for Ion Torrent NGS 1-16 Kit
23. Barcode Adaptors for Ion Torrent NGS 1-96 Kit
技术特点
· 无需脱蜡和预处理
· 单张FFPE可建库
· 一管试剂制备(文库)
· 超简化的FFPE NGS工作流程
· 接头兼容Ion torrent和Illumina平台
NGS文库制备试剂试剂优势
· 0.125-0.25张FFPE切片可完成建库,切片样本量要求小
· 免脱蜡,直提切片DNA
· 无需提取和纯化DNA
· 手工操作时间短(10min)
· 步骤简单,高效,成本低
· 靶向扩增和测序
· 也同时适用于新鲜组织样本
· 可用于多种NGS测序平台